38. Yeast Extract-Medium Extract Agar Ⅱ (酵母膏、麦芽膏琼脂 Ⅱ)
Yeast extract (酵母膏) 4g Mall extract (麦芽膏) 10g
Glucose (葡萄糖) 4g Agar (琼脂) 17g
Distilled water (蒸馏) 1000ml pH 7.3
39. Oatemeal Agar (燕麦粉琼脂)
Oatemeal(infusion)[燕麦粉浸液] 20g Agar (琼脂) 18%
Trace element solution (迹量盐溶液)1ml
Distilled water up to (用蒸馏水补充至1000毫升) 1000ml pH 7.2
[Note]:Trace element solution (迹量盐溶液):
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g Distilled water (蒸馏水) 100ml
40. Calcium Malate Agar (苹果酸钙琼脂)
Calium malate (苹果酸钙) 10g K2HPO4 0.5g
NH4Cl 0.5g Glycerol (甘油) 10g
Agar (琼脂) 15g Distilled water (蒸馏水) 1000ml
45. Pea Agar (豌豆琼脂)
Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5%
NaCl 0.5% Agar (琼脂) 2.0%
Pea infusion (counted by NH2) [豌豆浸汁(以NH2计)] 12-14 mg∕100
pH (before sterilization) [消前] 7.2-7.5
[Note]: Preparetion of pea infusion (豌豆浸汁的制法) :
Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition . (称取白豌豆900克,置于5公升广口瓶中,加自来水3公升,用硫酸调pH至4.3-4.5,再加氯仿30毫升(防腐),加胶塞并用纱布扎口(防止冲脱),放37℃恒温室7-10天。取出用滤纸过滤,滤液煮沸20分钟,以除氯仿,并再次以滤纸过滤,除沉淀。滤液分装,于120℃30分钟灭菌。后冷藏备用。)
The standard quality of pea infusion as following (豌豆浸汁质量标准):
Aminonitrogen(氨基酸): 160-200 mg∕100ml
Soluble phosphoruse(溶磷): 400-600 μg∕ml
Color(色泽): auratus,transparent (金黄、透明)